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KMID : 0545120010110010065
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 1 p.65 ~ p.71
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Cloning and Sequencing of the β-Amylase Gene from Paenibacillus sp. and Its Expression in Saccharomyces cerevisiae
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Jeong, Tae Hee
Kim, Hee Ok/Park, Jeong Nam/Lee, Hye Jin/Shin, Dong Jun/Lee, HwangHee Blaise/Chun, Soon Bai
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Abstract
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A gene from Paenibacillus sp. KCTC 8848P encoding β-amylase was cloned and expressed in Escherichia coli. The Paenibacillus β-amylase gene consisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosomebinding site, GGAGG, was located 10bp upstream from the TTG initiation codon. The deduced amino acid sequence of the β-amylase gene had a 95% similarity to the β-amylase of Bacillus firmus. The β-amylase gene was introduced into wildtype strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.
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